Mus musculus |
DRX122056 |
DRP004212 |
10090 |
bone marrow |
treatment: cultured in DMEM for 5h |
NEB |
NA |
NA |
Austrofundulus limnaeus |
SRX2562350 |
SRP058458 |
52670 |
Whole Embryo |
tissue: Whole Embryo |
Illumina_2 |
NA |
NA |
Caenorhabditis elegans |
SRX2826562 |
SRP107241 |
6239 |
Whole worm cell lysate, Gravid Adult |
genotype: alg-5(tm1163) I; alg-5::3xHA::alg-5 II |
Illumina |
NA |
extract_protocol: Small RNAs (18-28-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase or Tobacco Alkaline Phosphatase to facilitate capture of 22G-RNAs, followed by ligation of preadenylated 3’ adapters and non-adenylated 5’ adapters. Adapter-ligated small RNAs were size selected at each ligation step from 12-15% polyacrylamide gels. Adapter-bound small RNAs were reverse transcribed and then amplified using PCR. PCR products (~136-146 bp) were size selected from 10% polyacrylamide gels |
Caenorhabditis elegans |
SRX2826561 |
SRP107241 |
6239 |
Whole worm cell lysate, Gravid Adult |
genotype: alg-5(tm1163) I |
Illumina |
NA |
extract_protocol: Small RNAs (18-28-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase or Tobacco Alkaline Phosphatase to facilitate capture of 22G-RNAs, followed by ligation of preadenylated 3’ adapters and non-adenylated 5’ adapters. Adapter-ligated small RNAs were size selected at each ligation step from 12-15% polyacrylamide gels. Adapter-bound small RNAs were reverse transcribed and then amplified using PCR. PCR products (~136-146 bp) were size selected from 10% polyacrylamide gels |
Caenorhabditis elegans |
SRX2826560 |
SRP107241 |
6239 |
Whole worm cell lysate, Gravid Adult |
genotype: N2 |
Illumina |
NA |
extract_protocol: Small RNAs (18-28-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase or Tobacco Alkaline Phosphatase to facilitate capture of 22G-RNAs, followed by ligation of preadenylated 3’ adapters and non-adenylated 5’ adapters. Adapter-ligated small RNAs were size selected at each ligation step from 12-15% polyacrylamide gels. Adapter-bound small RNAs were reverse transcribed and then amplified using PCR. PCR products (~136-146 bp) were size selected from 10% polyacrylamide gels |
Caenorhabditis elegans |
SRX2826567 |
SRP107241 |
6239 |
Whole worm cell lysate, Late L4 |
genotype: alg-5(ram-2) I |
Illumina |
NA |
extract_protocol: Small RNAs (18-28-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase or Tobacco Alkaline Phosphatase to facilitate capture of 22G-RNAs, followed by ligation of preadenylated 3’ adapters and non-adenylated 5’ adapters. Adapter-ligated small RNAs were size selected at each ligation step from 12-15% polyacrylamide gels. Adapter-bound small RNAs were reverse transcribed and then amplified using PCR. PCR products (~136-146 bp) were size selected from 10% polyacrylamide gels |
Caenorhabditis elegans |
SRX2826566 |
SRP107241 |
6239 |
Whole worm cell lysate, Late L4 |
genotype: N2 |
Illumina |
NA |
extract_protocol: Small RNAs (18-28-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase or Tobacco Alkaline Phosphatase to facilitate capture of 22G-RNAs, followed by ligation of preadenylated 3’ adapters and non-adenylated 5’ adapters. Adapter-ligated small RNAs were size selected at each ligation step from 12-15% polyacrylamide gels. Adapter-bound small RNAs were reverse transcribed and then amplified using PCR. PCR products (~136-146 bp) were size selected from 10% polyacrylamide gels |
Caenorhabditis elegans |
SRX2826565 |
SRP107241 |
6239 |
Whole worm cell lysate, Late L4 |
genotype: alg-5(ram-1[GFP::3xFLAG::alg-5 + loxP]) I |
Illumina |
NA |
extract_protocol: Small RNAs (18-28-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase or Tobacco Alkaline Phosphatase to facilitate capture of 22G-RNAs, followed by ligation of preadenylated 3’ adapters and non-adenylated 5’ adapters. Adapter-ligated small RNAs were size selected at each ligation step from 12-15% polyacrylamide gels. Adapter-bound small RNAs were reverse transcribed and then amplified using PCR. PCR products (~136-146 bp) were size selected from 10% polyacrylamide gels |
Caenorhabditis elegans |
SRX2826564 |
SRP107241 |
6239 |
Whole worm cell lysate, Late L4 |
genotype: alg-5(ram-1[GFP::3xFLAG::alg-5 + loxP]) I |
Illumina |
NA |
extract_protocol: Small RNAs (18-28-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase or Tobacco Alkaline Phosphatase to facilitate capture of 22G-RNAs, followed by ligation of preadenylated 3’ adapters and non-adenylated 5’ adapters. Adapter-ligated small RNAs were size selected at each ligation step from 12-15% polyacrylamide gels. Adapter-bound small RNAs were reverse transcribed and then amplified using PCR. PCR products (~136-146 bp) were size selected from 10% polyacrylamide gels |
Homo sapiens |
SRX12265127 |
SRP337938 |
9606 |
Plasma |
sample type: Normal - Cancer-free |
other |
NA |
NA |
Homo sapiens |
SRX12265125 |
SRP337938 |
9606 |
Plasma |
sample type: Normal - Cancer-free |
other |
NA |
NA |
Caenorhabditis elegans |
SRX23442614 |
SRP486660 |
6239 |
whole animals |
tissue: whole animals |
NEB |
NA |
NA |
Caenorhabditis elegans |
SRX23442613 |
SRP486660 |
6239 |
whole animals |
tissue: whole animals |
NEB |
NA |
NA |
Homo sapiens |
SRX12265123 |
SRP337938 |
9606 |
Plasma |
sample type: Normal - Cancer-free |
other |
NA |
NA |
Homo sapiens |
SRX8804169 |
SRP273213 |
9606 |
plasma |
tissue: plasma |
adapter_trimmed |
NA |
NA |