Austrofundulus limnaeus |
SRX2562340 |
SRP058458 |
52670 |
Whole Embryo |
tissue: Whole Embryo |
Illumina |
NA |
NA |
Caenorhabditis elegans |
SRX2826552 |
SRP107241 |
6239 |
Whole worm cell lysate, Gravid Adult |
genotype: alg-2(ok304) II; alg-2::HA::alg-2 IV |
Illumina |
NA |
extract_protocol: Small RNAs (18-28-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase or Tobacco Alkaline Phosphatase to facilitate capture of 22G-RNAs, followed by ligation of preadenylated 3’ adapters and non-adenylated 5’ adapters. Adapter-ligated small RNAs were size selected at each ligation step from 12-15% polyacrylamide gels. Adapter-bound small RNAs were reverse transcribed and then amplified using PCR. PCR products (~136-146 bp) were size selected from 10% polyacrylamide gels |
Arabidopsis thaliana |
SRX8554598 |
SRP267491 |
3702 |
rosette leaves |
molecule subtype: Small RNA |
other |
NA |
NA |
Caenorhabditis elegans |
SRX2826551 |
SRP107241 |
6239 |
Whole worm cell lysate, Gravid Adult |
genotype: alg-1::HA::alg-1 IV; alg-1(gk214) X |
Illumina |
NA |
extract_protocol: Small RNAs (18-28-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase or Tobacco Alkaline Phosphatase to facilitate capture of 22G-RNAs, followed by ligation of preadenylated 3’ adapters and non-adenylated 5’ adapters. Adapter-ligated small RNAs were size selected at each ligation step from 12-15% polyacrylamide gels. Adapter-bound small RNAs were reverse transcribed and then amplified using PCR. PCR products (~136-146 bp) were size selected from 10% polyacrylamide gels |
Arabidopsis thaliana |
SRX8554597 |
SRP267491 |
3702 |
rosette leaves |
molecule subtype: Small RNA |
other |
NA |
NA |
Caenorhabditis elegans |
SRX2826550 |
SRP107241 |
6239 |
Whole worm cell lysate, Gravid Adult |
genotype: N2 |
Illumina |
NA |
extract_protocol: Small RNAs (18-28-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase or Tobacco Alkaline Phosphatase to facilitate capture of 22G-RNAs, followed by ligation of preadenylated 3’ adapters and non-adenylated 5’ adapters. Adapter-ligated small RNAs were size selected at each ligation step from 12-15% polyacrylamide gels. Adapter-bound small RNAs were reverse transcribed and then amplified using PCR. PCR products (~136-146 bp) were size selected from 10% polyacrylamide gels |
Arabidopsis thaliana |
SRX8554596 |
SRP267491 |
3702 |
rosette leaves |
molecule subtype: Small RNA |
other |
NA |
NA |
Arabidopsis thaliana |
SRX8554595 |
SRP267491 |
3702 |
rosette leaves |
molecule subtype: Small RNA |
other |
NA |
NA |
Caenorhabditis elegans |
SRX2826556 |
SRP107241 |
6239 |
Whole worm cell lysate, Gravid Adult |
genotype: alg-1::HA::alg-2 IV; alg-1(gk214) X |
Illumina |
NA |
extract_protocol: Small RNAs (18-28-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase or Tobacco Alkaline Phosphatase to facilitate capture of 22G-RNAs, followed by ligation of preadenylated 3’ adapters and non-adenylated 5’ adapters. Adapter-ligated small RNAs were size selected at each ligation step from 12-15% polyacrylamide gels. Adapter-bound small RNAs were reverse transcribed and then amplified using PCR. PCR products (~136-146 bp) were size selected from 10% polyacrylamide gels |
Caenorhabditis elegans |
SRX2826555 |
SRP107241 |
6239 |
Whole worm cell lysate, Gravid Adult |
genotype: alg-2(ok304) II; alg-2::HA::alg-2 IV |
Illumina |
NA |
extract_protocol: Small RNAs (18-28-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase or Tobacco Alkaline Phosphatase to facilitate capture of 22G-RNAs, followed by ligation of preadenylated 3’ adapters and non-adenylated 5’ adapters. Adapter-ligated small RNAs were size selected at each ligation step from 12-15% polyacrylamide gels. Adapter-bound small RNAs were reverse transcribed and then amplified using PCR. PCR products (~136-146 bp) were size selected from 10% polyacrylamide gels |
Caenorhabditis elegans |
SRX2826554 |
SRP107241 |
6239 |
Whole worm cell lysate, Gravid Adult |
genotype: alg-1::HA::alg-1 IV; alg-1(gk214) X |
Illumina |
NA |
extract_protocol: Small RNAs (18-28-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase or Tobacco Alkaline Phosphatase to facilitate capture of 22G-RNAs, followed by ligation of preadenylated 3’ adapters and non-adenylated 5’ adapters. Adapter-ligated small RNAs were size selected at each ligation step from 12-15% polyacrylamide gels. Adapter-bound small RNAs were reverse transcribed and then amplified using PCR. PCR products (~136-146 bp) were size selected from 10% polyacrylamide gels |
Arabidopsis thaliana |
SRX8554589 |
SRP267491 |
3702 |
rosette leaves |
molecule subtype: Small RNA |
other |
NA |
NA |
Caenorhabditis elegans |
SRX2826553 |
SRP107241 |
6239 |
Whole worm cell lysate, Gravid Adult |
genotype: alg-1::HA::alg-2 IV; alg-1(gk214) X |
Illumina |
NA |
extract_protocol: Small RNAs (18-28-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase or Tobacco Alkaline Phosphatase to facilitate capture of 22G-RNAs, followed by ligation of preadenylated 3’ adapters and non-adenylated 5’ adapters. Adapter-ligated small RNAs were size selected at each ligation step from 12-15% polyacrylamide gels. Adapter-bound small RNAs were reverse transcribed and then amplified using PCR. PCR products (~136-146 bp) were size selected from 10% polyacrylamide gels |
Arabidopsis thaliana |
SRX8554588 |
SRP267491 |
3702 |
rosette leaves |
molecule subtype: Small RNA |
other |
NA |
NA |
Caenorhabditis elegans |
SRX2826559 |
SRP107241 |
6239 |
Whole worm cell lysate, Gravid Adult |
genotype: alg-2(ok304) II |
Illumina |
NA |
extract_protocol: Small RNAs (18-28-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase or Tobacco Alkaline Phosphatase to facilitate capture of 22G-RNAs, followed by ligation of preadenylated 3’ adapters and non-adenylated 5’ adapters. Adapter-ligated small RNAs were size selected at each ligation step from 12-15% polyacrylamide gels. Adapter-bound small RNAs were reverse transcribed and then amplified using PCR. PCR products (~136-146 bp) were size selected from 10% polyacrylamide gels |