Name | Exp | Study | TaxonID | SampleTag | SampleTag2 | LibraryInf | Disease | Method |
---|---|---|---|---|---|---|---|---|
Caenorhabditis elegans | SRX2826558 | SRP107241 | 6239 | Whole worm cell lysate, Gravid Adult | genotype: alg-1(gk214) X | Illumina | NA | extract_protocol: Small RNAs (18-28-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase or Tobacco Alkaline Phosphatase to facilitate capture of 22G-RNAs, followed by ligation of preadenylated 3’ adapters and non-adenylated 5’ adapters. Adapter-ligated small RNAs were size selected at each ligation step from 12-15% polyacrylamide gels. Adapter-bound small RNAs were reverse transcribed and then amplified using PCR. PCR products (~136-146 bp) were size selected from 10% polyacrylamide gels |
Caenorhabditis elegans | SRX2826557 | SRP107241 | 6239 | Whole worm cell lysate, Gravid Adult | genotype: N2 | Illumina | NA | extract_protocol: Small RNAs (18-28-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase or Tobacco Alkaline Phosphatase to facilitate capture of 22G-RNAs, followed by ligation of preadenylated 3’ adapters and non-adenylated 5’ adapters. Adapter-ligated small RNAs were size selected at each ligation step from 12-15% polyacrylamide gels. Adapter-bound small RNAs were reverse transcribed and then amplified using PCR. PCR products (~136-146 bp) were size selected from 10% polyacrylamide gels |
Homo sapiens | SRX12265137 | SRP337938 | 9606 | Plasma | sample type: Normal - Cancer-free | other | NA | NA |
Austrofundulus limnaeus | SRX2562339 | SRP058458 | 52670 | Whole Embryo | tissue: Whole Embryo | Illumina | NA | NA |
Homo sapiens | SRX12265135 | SRP337938 | 9606 | Plasma | sample type: Normal - Cancer-free | other | NA | NA |
Austrofundulus limnaeus | SRX2562338 | SRP058458 | 52670 | Whole Embryo | tissue: Whole Embryo | Illumina_2 | NA | NA |
Homo sapiens | SRX12265133 | SRP337938 | 9606 | Plasma | sample type: Normal - Cancer-free | other | NA | NA |
Homo sapiens | SRX12265131 | SRP337938 | 9606 | Plasma | sample type: Normal - Cancer-free | other | NA | NA |
Austrofundulus limnaeus | SRX2562355 | SRP058458 | 52670 | Whole Embryo | tissue: Whole Embryo | Illumina | NA | NA |
Homo sapiens | SRX12265119 | SRP337938 | 9606 | Plasma | sample type: Normal - Cancer-free | other | NA | NA |
Homo sapiens | SRX12265117 | SRP337938 | 9606 | Plasma | sample type: Normal - Cancer-free | other | NA | NA |
Austrofundulus limnaeus | SRX2562356 | SRP058458 | 52670 | Whole Embryo | tissue: Whole Embryo | Illumina_2 | NA | NA |
Mus musculus | DRX122058 | DRP004212 | 10090 | bone marrow | treatment: cultured in DMEM for 0h | NEB | NA | NA |
Mus musculus | DRX122057 | DRP004212 | 10090 | bone marrow | treatment: cultured in DMEM for 2h | NEB | NA | NA |
Caenorhabditis elegans | SRX2826563 | SRP107241 | 6239 | Whole worm cell lysate, Gravid Adult | genotype: alg-5(tm1163) I; alg-5::3xHA::alg-5 II | Illumina | NA | extract_protocol: Small RNAs (18-28-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase or Tobacco Alkaline Phosphatase to facilitate capture of 22G-RNAs, followed by ligation of preadenylated 3’ adapters and non-adenylated 5’ adapters. Adapter-ligated small RNAs were size selected at each ligation step from 12-15% polyacrylamide gels. Adapter-bound small RNAs were reverse transcribed and then amplified using PCR. PCR products (~136-146 bp) were size selected from 10% polyacrylamide gels |