tRNA annotations can be obtained parsing a species out of the genomic tRNA database (http://gtrnadb.ucsc.edu/ ).
Test with: Homo sapiens
If you use this data please cite: Chan, P.P., Lowe, T.M. (2009) GtRNAdb: A database of transfer RNA genes detected in genomic sequence Nucl. Acids Res. 37(Database issue):D93-D97.